(PR071) Biocompatibility of Various Bioceramic-Based Root Canal Sealers: An In Vitro Study

Objective: This study aimed to compare the biocompatibility of various bioceramic-based root canal sealers.

Methods: Four recently introduced bioceramic-based root canal sealers—Neo Sealer Flo (NSF), RE-GEN™ Bioactive EndoSealer (RGB), Bio-C Sealer Ion⁺ (BIC), and EndoSequence BC Sealer (EBS)—were evaluated. Their cytotoxicity was assessed using the Alamar Blue reduction assay in human periodontal ligament fibroblasts (hPDLFs) and compared to a control group containing only cells. Additionally, the release of pro-inflammatory cytokines, interleukin-6 (IL-6) and interleukin-8 (IL-8), from hPDLFs was measured to evaluate the inflammatory response to the sealers. Data was analyzed using a one-way analysis of variance (ANOVA), followed by Tukey’s post hoc test, with significance set at 95%.

Results: All bioceramic-based sealers demonstrated high cell viability, comparable to the control group, indicating low cytotoxicity. In the ELISA test, RGB and EBS showed significantly higher IL-8 expression compared to the other sealers, while there was no significant difference in IL-6 expression among the groups.

Conclusion: This study demonstrated favorable in vitro biocompatibility for the evaluated bioceramic-based root canal sealers, with all materials showing minimal cytotoxicity. However, differences in cytokine release suggest varying levels of inflammatory responses, which may warrant further investigation.