(PR107) Evaluation of an Endodontic Irrigation Protocol Based on an Enzymatic and Hyperosmotic Solution on a Mixed Biofilm

Objective: To evaluate an endodontic irrigation protocol based on an enzymatic and hyperosmotic solution on a mixed biofilm.

Methods: 36 roots were selected and standardized. The canal was patented with K files and a Haapasalo protocol was performed for disinfection. Next, the mixed biofilm of E. Faecalis and C. Albicans, was formed by a static method. Subsequently, the irrigating solutions were prepared, the enzymatic solution was prepared from enzyme from Trichoderma reesei and the hyperosmotic solution was prepared based on the proposal of Dr. Mónica Rojas in 2020. The instrumentation was carried out: using the solutions to be tested. Finally, the evaluation was carried out by counting Colony Forming Units (CFU) and in a Scanning Electron Microscope (SEM).

Results: Samples were taken before and after instrumentation. Dilutions were made on McFarland scale and a culture for UFC was performed where we found a small antimicrobial activity by the enzymatic solution, but it doesn’t show significant difference, the hyperosmotic solution did not show activity on the biofilm and the NaClO group showed a better activity with a significant difference. Finally, for the observation in the SEM the results are very comparable with the UFC. Biofilm is observed on the dentin surface for the enzymatic and hyperosmotic solutions while in the NaClO group the semipermeable tubules are observed.

Conclusions: The enzymes had little effect on the mature biofilm polymers and the hyperosmotic solution managed to eliminate only microorganisms in the platonic state, while NaClO had good results on the formed biofilm.