(PR094) Cytotoxicity and Genotoxicity of Triton All-In-One Irrigation Solution - An In Vitro Study

Objective: The success of endodontic therapy depends on eradicating microorganisms within the root canal system and preventing reinfection. Traditional irrigants like sodium hypochlorite (NaOCl) and Ethylenediaminetetraacetic Acid (EDTA) provide broad-spectrum antimicrobial activity; however, they carry risks, including cytotoxicity. The Triton All-in-One Solution is a newer irrigation product that combines approximately 4% NaOCl with a blend of gentle chelators and surfactants, aiming to simplify the disinfection protocol by reducing procedural steps and potentially enhancing antimicrobial efficacy. This study compares the cytotoxic and genotoxic effects of the Triton All-in-One Solution with those of traditional NaOCl/EDTA protocols in root canal treatment.

Methods: To assess cytotoxicity, human periodontal ligament fibroblasts were exposed to NaOCl at concentrations of 1.5%, 2.5%, 4%, and 6%, as well as to 17% EDTA and the Triton All-in-One Solution. Cell viability was measured using the CellTox Green assay at 1, 3, 5, and 10 minutes, with lysis buffer as a positive control and phosphate-buffered saline as a negative control.

Results: There was no statistically significant difference in the cytotoxic effects of Triton across various time points or when compared with all NaOCl concentrations (p > 0.05). Two-way ANOVA with Tukey’s test indicated no significant difference in cytotoxicity between Triton and NaOCl concentrations at any time point. Triton appears to have a cytotoxic profile similar to that of 6% NaOCl.

Conclusion: The Triton All-in-One Solution shows cytotoxicity comparable to 6% NaOCl, providing an additional alternative for root canal disinfection. Further testing is underway to assess its genotoxic potential.