(PR099) Disinfection Efficacy of Different Irrigation Systems Combined With a Hydrodynamic Cavitation System (ODNE CLEAN)

Objective: This study aims to test the efficacy of three different irrigation systems when combined with hydrodynamic cavitation using saline against Enterococcus faecalis biofilm in root canal size (20/.04) model. Materials &

Methods: Distobuccal canals of maxillary first molars were instrumented to size 20/.04 and autoclaved. Canals were then incubated with E. faecalis for 21 days. Teeth were randomly divided into 6 groups (15 teeth/group) standard needle irrigation (SNI), ultrasonic activated irrigation (UAI), or LEAP (L) with or without the use of Odne Clean. Samples were taken prior to (S1) and after (S2) disinfection. Five teeth were selected randomly from each group and sectioned at the coronal, middle and apical third, stained using live/dead bacteria stain and analyzed using confocal laser scanning microscopy (CLSM). Bacterial viability and vitality were assessed through colony forming unit (CFU) counts, and confocal laser scanning microscopy.

Results: All S2 samples showed significant bacterial reduction compared to S1 (p < 0.05). Quantitative analysis demonstrated a bacterial reduction of: SNI - 98.6%; SNI with ODNE CLEAN - 99.43%; UAI - 99.2%; UAI with ODNE CLEAN - 98.34%; and L - 97.9%; L with ODNE CLEAN - 99.45%. CLSM demonstrated various bacterial penetration capability among the coronal, middle and apical segments of the canals.

Conclusion: The integration of the Odne Clean system with SNI, UAI and LEAP laser systems significantly enhances the penetration depth and disinfection efficacy within dentinal tubules.