(PR082) Comparative Histological Study of Pulp Debridement Using Various Irrigation Techniques

Methods: Twenty freshly extracted teeth diagnosed with symptomatic irreversible pulpitis and symptomatic apical periodontitis were collected and divided into five groups (n=5): Passive Ultrasonic Irrigation (PUI), OdneClean and OdneClean using saline, SWEEPS, and EdgePro Laser. Working length was determined for each tooth, and cleaning and shaping were performed to a final size of 20/04. Final irrigation was carried out according to the specific protocol for each group. Following treatment, the samples were fixed in formalin and decalcified for histological processing. Roots were sectioned into apical, middle, and cervical thirds. These sections were embedded in paraffin, 5 sections of 5 mm section were obtained from each segment and stained with H&E and examined under light microscopy. Histological images were analyzed to quantify pulp remnants using ImageJ software.

Results: All techniques were not capable to fully debride the pulp tissue from the root canal system, specially from the difficult to reach areas. The apical and middle thirds demonstrate differences when compared to the coronal third, though these differences are not statistically significant.

Conclusion: This study seeks to identify the most efficient irrigation method in promoting optimal cleaning and disinfection for improved endodontic outcomes. While the initial findings suggest limited variability in pulpal dissolution based on groupings, the observed trends may warrant further investigation with a bigger sample size.