(PR068) Assessment of the Physicochemical and Biological Properties of New Calcium Silicate-based Endodontic Sealers

Objective: To compare the physicochemical and biological properties of AH Plus Jet (AHP), BioRoot Flow (BRF), EndoCeramic (EC) and ZenSeal (ZS).

Methods: Setting time, solubility, pH, radiopacity and flow were evaluated following ISO 6876. Biocompatibility was evaluated through XTT using human periodontal ligament fibroblasts treated with 24- and 48h-extract media. mRNA expression levels of TNF, TGFβ, IL10, COL1, ALP, RANKL and OPG were evaluated using RT-qPCR. ANOVA and Tukey's were used for physicochemical and Kruskal-Wallis and Dunn's multiple comparison tests used for cytotoxicity. P≤0.05 and fold-change >2 were considered statistically significant.

Results: EC had the shortest initial and final setting times (P < 0.05). BRF had the highest solubility and pH, meanwhile EC had the lowest flow, and AHP had the highest radiopacity (P < 0.05). EC-, BRF- and ZS-treated cells showed higher viability than AHP-treated cells (P < 0.01), and BRF-treated cells showed lower viability than ZS and EC (P < 0.01). No significant differences were observed for mRNA expression of IL10 and OPG for all sealers ( < 2 fold-change). RANKL was upregulated in cells treated with 24h-ZS ( >2 fold-change). COL1 and TNF were upregulated in cells treated with 24h-AHP and -BRF. TNF was also upregulated in cells treated with 48h-BRF ( >2 fold-change). TGFβ was upregulated in cells treated with 48h-AHP and -BRF ( >2 fold-change). ALP was upregulated in cells treated with 24h-ZS, and 48h of all sealers ( >2 fold-change).

Conclusion: Calcium silicate-based sealers (BRF, EC and ZS) presented adequate physicochemical and biological properties, except for solubility, which may still be a clinical concern.