(PR130) Physico-Chemical and Biological Properties of a Prototype Bioceramic Sealer

Objectives: This study investigated the properties of a prototype bioceramic sealer (PS), AH Plus (AHP), EndoSequence BC Sealer (BC), and EndoCeramic Bioceramic Sealer (EC).

Methods: Setting time, radiopacity, flow, solubility, calcium ion release, and pH were evaluated following ISO guidelines. Antibacterial effect was tested against Enterococcus faecalis, and cytotoxicity tested using XTT cell viability assay. Leakage was assessed by microscopic evaluation of obturated teeth following 7-day ink suspension. One-and two-way ANOVA with Tukey post-hoc tests were used for statistical analysis (a=0.05)

Results: AHP and EC set faster than PS or BC, and BC was less radiopaque than AHP and EC, with no significant differences in radiopacity among other sealers (p>0.05). AHP had higher flow compared to PS and EC, but there was no significant difference between PS, EC, and BS. BC and PS were significantly more soluble than AHP and EC. BC, EC and PS all had significantly more calcium release compared to AHP with no significant differences between them. EC and PS showed lower pH compared to AHP and BC (p < 0.05). BC maintained highest pH overall (p < 0.05). All sealers eliminated E. faecalis after 24 hours of contact. No significant differences were observed between sealer-treated HPDL cells and negative control (p>0.05), with all sealers displaying low cytotoxicity. There were no significant differences in microleakage between AHP, BC, and EC (p < 0.05). PS showed significantly less microleakage against EC (p < 0.05).

Conclusion: Overall, PS may require further refinement to match desirable qualities of currently available sealers.