(PR016) Ex-Vivo Evaluation of Bacteria Biofilm, Smear Layer, and Debris Removal Efficacy of a Hydro-Dynamic Cavitation System with Physiological Saline Using a New Artificial Root Canal Model

Objective: To evaluate bacteria biofilm, smear layer and debris removal efficacy of a hydro-dynamic cavitation system with physiological saline using a new artificial root canal model.

Methods: 60 dentin discs were used to grow 3-week-old bacterial biofilms, while smear layer and debris were produced on fifteen dentin discs. The 60 artificial root canals with biofilm-covered dentin discs were divided 6 groups: control, needle with physiological saline, ultrasonic with physiological saline, Odne^TM Clean with physiological saline, needle with 3% NaOCl, and ultrasonic with 3% NaOCl. Biofilm removal efficacy was evaluated using confocal laser scanning microscopy. The fifteen artificial root canals with smear layer and debris-covered dentin discs were divided 3 groups: control, Odne Clean with physiological saline, and 5% NaOCl followed by 17% EDTA. Smear layer and debris removal efficacy was evaluated using scanning electron microscopy.

Results: Odne Clean with physiological saline performed significantly better than needle irrigation or ultrasonic activation with physiological saline (p < 0.05). Done Clean with physiological saline removed biofilms as effectively as needle or ultrasonic with 3% NaOCl (p>0.05). Additionally, 5% NaOCl followed by 17% EDTA removed the smear layer significantly better than Odne Clean with physiological saline (p < 0.05). However, Odne Clean with physiological saline removed debris as effectively as 5% NaOCl followed by 17% EDTA (p>0.05).

Conclusions: Odne Clean can effectively remove bacteria biofilm and debris from dentin surface but cannot effectively remove the smear layer. Utilizing Odne Clean during the final irrigation stage may enhance the antibacterial and cleaning efficacy of the root canal.