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Assessment of New Technology Such as Devices and Materials

OR03 - Comparative Efficacy of Multisonic Ultracleaning System (MUS) GentleWave® system Versus Er:YAG laser system in Reducing Bacterial Biofilm within Root Canals

Wednesday, April 2, 2025
10:30 AM – 10:45 AM EST
Location: Hynes Convention Center, Floor 3, Room 309

    Presenter(s)

  • Malik Algrouz, D.M.D.

    Endodontic Resident
    University of Illinois at Chicago
    Chicago, Illinois

    Disclosure information not submitted.

    • Co-Author(s)

  • Qian Xie, D.D.S., M.S., Ph.D.

    Associate Professor
    University of Illinois at Chicago
    Chicago, Illinois

    Disclosure information not submitted.

  • SJ

    Shawn Jung, D.M.D., M.S.

    Clinical Assistant Professor
    University of Illinois at Chicago
    Chicago, Illinois

    Disclosure information not submitted.

  • Mohamed Fayad, D.D.S., M.S., Ph.D.

    Clinical Associate Professor
    University of Illinois at Chicago
    University of Illinois at Chicago
    Chicago, Illinois

    Disclosure(s): No financial relationships to disclose

Disclosure(s):
Mohamed Fayad, D.D.S., M.S., Ph.D.: No financial relationships to disclose
Objective: This in vitro study aimed to evaluate the efficacy of biofilm removal using two advanced irrigation systems: the Multisonic Ultracleaning System (MUS) GentleWave® with the CleanFlow™ handpiece, and the erbium-doped yttrium-aluminum-garnet (Er:YAG) laser (LightWalker® Fotona) for Laser-Activated Irrigation (LAI). The modes used in LAI included Photon Induced Photo-acoustic Streaming (PIPS) and Shock Wave Enhanced Emission Photo-acoustic Streaming (SWEEPS). Materials and

Methods: Fifty extracted human mandibular molars meeting the inclusion criteria were collected, processed, and sterilized following Institutional Review Board (IRB) approval. The teeth were inoculated with Enterococcus faecalis and cultured for 21 days to develop biofilms. The biofilm formation in the canals was sampled and confirmed with confocal laser microscopy. After incubation, the samples were randomly divided into four groups (n=12): Conventional Needle Irrigation (CNI), MUS system, Er:YAG SWEEPS, and Er:YAG PIPS. The teeth were instrumented to size 25/V06 and irrigated according to the respective treatment protocols. Bacterial samples were collected pre- and post-instrumentation. Data were analyzed using an analysis of variance with a significance level of p<.05, followed by Dunnett’s and Tukey's tests.

Results: Er:YAG SWEEPS reduced E. faecalis significantly more than CNI and MUS systems (p < 0.05). There was no statistically significant difference in bacterial reduction when comparing MUS and PIPS (p>0.05).

Conclusion: Enhanced irrigation techniques might improve antimicrobial action against E. faecalis biofilms. The Er:YAG SWEEPS proved to be more effective than Er:YAG PIPS and MUS system within the limitations of the current study.